| RIOK1

Serine/threonine-protein kinase RIO1 is an enzyme that is encoded by the RIOK1 gene.RIOK1 is an atypical protein, which exists in most archaea and eukaryotes. It belongs to the serine/threonine-specific protein kinase family.It has been intensely studied to understand the maturation they promote on small ribosomal subunits (SSU). It is suggested that over-expression or mutations of the RIOK 1 gene may cause mis-regulation of its network (in metazoans - large signaling network at the protein and gene levels via which it stimulates or restricts growth and division in response to nutrient availability).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.32	1.89	1.4	0.97	0.58	0.37	0.22	0.07
Corrected OD	2.25	1.82	1.33	0.9	0.51	0.3	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human RIOK1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human RIOK1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	82-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	100